A REVIEW ON DEVELOPMENT AND ESTIMATION OF PHARMACEUTICAL FORMULATION BY GAS CHROMATOGRAPHY(C-18)

Gas chromatography is a sensitive, accurate, reproducible, quantitative and versatile tool well adapted for the analysis of complex mixtures. Analytical method development plays a crucial role in ensuring the quality and safety of pharmaceutical formulations. In the present work a gas chromatographic method was developed and optimised for the estimation of a pharmaceutical formulation. The study focused on systemic optimization of chromatographic conditions to achieve effective separation, reproducible retention, behaviour and reliable quantification of the analyte. Method development involved the selection of appropriate column characteristics, carrier gas flow conditions and temperature programming to enhance sensitivity and peak resolution. The development method was validation in accordance with ICH guidelines validation parameters such as linearity accuracy, precision limit of detection, limit of quantification and robustness were evaluated and found to be within acceptable limits. The proposed gas chromatography method demonstrate satisfactory performance for the estimation of pharmaceutical formulations and can be effectively applied for routine quality control analysis.

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